Field Evaluation and Enzyme-Linked Immunosorbent Assay Detection of Potato Leaf Roll Virus, Potato Virus X and Potato Virus Y in Potato Germplasm
نویسندگان
چکیده
منابع مشابه
تعیین برخی خصوصیات بیولوژیکی و مولکولی potato leafroll virus و potato virus a در استان اصفهان
چکیده سیب زمینی یکی از مهم ترین محصولات زراعی در جهان می باشد. ویروس های متعددی این گیاه را تحت تاثیر قرار می دهند و باعث خسارت اقتصادی قابل توجهی می شوند. ویروس پیچیدگی برگ سیب زمینی potato leafroll virus (plrv) عضو تیپ جنس polerovirus و خانواده luteoviridae و ویروس ای سیب زمینی potato virus a (pva) عضو تیپ جنس potyvirus از خانواده potyviridae شایع ترین ویروس های سیب زمینی می باشند که توسط ش...
15 صفحه اولErratum: The Effects of High Temperature on Infection by Potato virus Y, Potato virus A, and Potato leafroll virus
[This corrects the article on p. 321 in vol. 32, PMID: 27493607.].
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Field sweet potato plants showing virus-like symptoms, as stunting, leaf distortion, mosaic and chlorosis, were collected in southwest Portugal and tested for the presence of four potyviruses, sweet potato virus C (SPVC), sweet potato virus 2 (SPV2), sweet potato feathery mottle virus (SPFMV), sweet potato virus G (SPVG), and the crinivirus sweet potato chlorotic stunt virus (SPCSV). DsRNA frac...
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Background: The advent of recombinant DNA technology has facilitated heterologous expression of proteins from various sources in different host systems including Escherichia coli. If a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. Such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...
متن کاملDigoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens: potato spindle tuber viroid (PSTVd), potato virus Y (PVY), and potato leafroll virus (PLRV).
A molecular probe, p3POT, was constructed of PSTVd, PVY, PLRV cDNA fragments introduced into pUC18 vector. Sequencing of the inserts revealed that cloned fragments covered conservative parts of pathogenic genomes. Dot-blot hybridization of digoxigenin-labelled construct to crude extracts from plants infected with different potato viruses proved high sensitivity and specificity of the p3POT prob...
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ژورنال
عنوان ژورنال: Journal of Agricultural Science
سال: 2017
ISSN: 1916-9760,1916-9752
DOI: 10.5539/jas.v9n7p229